Abstract

TERFEZIA BOUDIERI AS SOURCES OF ANTITUMOR AND ANTIVIRAL AGENT

Dr. Amal A.I. Mekawey

ABSTRACT

Terfezia boudieri was used to study its ability to use as source of antitumor and antiviral agent. Six tumor cell lines and two viral cells (HSV-2 and VSV) tested for this investigation. Four extracts from T. boudieri were obtained by four solvents and SRB and MTT assays were occurred for measurement of Potential as antitumor and antiviral potency, respectively. Separation, purification and identification of most active compound were performed. Aqueous extract exhibited good results as antitumor agent whereas it exhibited IC50 against four cells lines HEP2, HCT116, MCF7 and CACO2 (3.6, 7.2, 8.4 and 9.2 [μm/ml], respectively) followed by chloroform extract (its IC50 against three cell lines) then acetone and methanol exhibited IC50 against only two cell lines as compared with 1 μm/ml of antitumor drug vinblastine. Also, four T. boudieri extracts showed moderateinhibitory activity on HSV-2 where 75%, 64%, 49% and 35% inhibitory activity at concentration 10μg/ml of aqueous, chloroform, acetone and methanol extracts, respectively and 70%, 33%, 32% and 50% inhibition of VSV at the same concentrations as compared to acyclovir standard. Methyl-8-acetoxy-1,2,-tetradecahydro-1,5-dihydroxylchrysene-2-carboxylate (MATDC) is the compound extracted from aqueous extract of Terfezia boudieri and exhibited high potency as antitumor and antiviral agent.

Keywords: Terfezia boudieri, antitumor, antiviral, SRP, MTT, HEPG2; HELA; MCF7; CACO; HCT116; HEP2, BHK, vinblastine, HSV-2; VSV and acyclovir, TLC.