Abstract

CYTOTOXIC EFFECT OF THE PURIFIED L-ASPARAGINASE FROM LOCALLY ISOLATE PSUDOMONAS PUTIDA ON HEP-2 TUMOR CELL LINE

Sahira Nsayef Muslim*, Alaa Naseer Mohammed Ali, Farooq Ibrahem mohammed, Baraa abdulhadi abdulhameed and Suhad Abbas Abid

ABSTRACT

L-asparaginase is an enzyme that catalyzes the conversion of L-asparagine to L-aspartate and ammonia. L-asparaginase has medical important in using it as chemotherapeutic for its anticarcinogenic potential. In this research a novel strain, Pseudomonas putida R2 was showed the ability to produce of L-asparaginase. This enzyme was purified to homogeneity with a recovery yield of 87.8% and 32 fold of purification by using ammonium sulfate at 50% saturation followed by ion exchange on DEAE-cellulose column and gel filtration on Sephadix G100 column. L-asparaginase purified from Pseudomonas putida at a concentration of 255 μg∕ml after 72h of exposure showed better toxicity on Hep-2 cell line in comparison with the control, and this result led to increase the benefit by using the L-asparaginase for the treatment of human larynx epidermoid carcinoma.

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