Abstract

BIOSYNTHESIS OF SILVER NANOPARTICLES USING NICOTIANA TOBACCUM LEAF EXTRACT

S. R. Kuchekar*, M. P. Patil and Sung-H Han

ABSTRACT

Plant extract from tobacco leaf was used for the synthesis of silver nanoparticles (AgNps) using the silver nitrate solution. AgNps were characterized by UV-Vis spectrophotometer, X-ray diffractometer (XRD), Scanning electron microscope (SEM) and Energy dispersive spectroscopy (EDX). The formation and stability of the reduced silver nanoparticles in the colloidal solution were monitored by UV-Vis. Spectrophotometer analysis. The mean particle diameter of silver nanoparticles was calculated from the XRD pattern according to the line width of the plane, refraction peak using the Scherrer’s equation. SEM determination of the brown colour stable sample showed the formation of silver nanoparticles and well dispersed nanoparticles could be seen in the samples treated with silver nitrate. EDX analysis gives elemental status that may be involved in the formationof nanoparticles. In the research article, we present a simple and eco- friendly bio-synthesis of silver nanoparticles using tobacco leaf extract as reducing agent.

Keywords: Biosynthesis, Tobacco leaf extract, Silver nanoparticles.