Abstract

INVITRO ANALYSIS OF PHYTOCHEMICAL AND ANTIUROLITHIATIC ACTIVITY OF VARIOUS EXTRACTS OF MELIA DUBIA LEAVES.

Vennila V* and Marthal Mariyal A.

ABSTRACT

Objective: To study the antiurolithiatic activity of various solvent extracts of Melia dubia leaves. Methods: The inhibition of in-vitro calcium oxalate crystal formation by various solvent extracts of M. dubia leaves were investigated by synthetic urine assay. In synthetic urine method, the calcium oxalate formation is induced by the addition of 0.01 M sodium oxalate solution and the inhibition percentage of the calcium oxalate crystals from synthetic urine at different concentrations of various solvent extracts of M. dubia Leaves (10, 50 and 100mg) was investigated by the time course measurement of turbidity changes due to the crystal nucleation and aggregation in the synthetic urine at 620nm by means of a spectrophotometer. Results: The aqueous, acetone and ethanol extracts of M. dubia leaves remarkably inhibits the crystal formation in dose dependent manner in the calcium oxalate urinary lithiasis. Conclusion: Acetone extract of M. dubia leaves showed maximum inhibitory effect on calcium oxalate crytallisation in synthetic urine than ethanol and water extracts. Thus acetone extract could be further analyzed in vivo and characterization of its active compound could lead to the discovery of a new drug for urolithiasis.

Keywords: Antiurolithiatic activity, Acetone extract, Melia dubia, Synthetic urine assay, Calcium oxalate crystallisation.