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A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF EFAVIRENZ IN PHARMACEUTICAL FORMULATION*S.Ramesh1, S.Alexandar2 and S.Muniyappan3
1Department of pharmaceutical analysis, J.K.K.M. College of pharmacy, Kumarapalayam, Erode, India. 2Department of pharmaceutical chemistry, JSS College of Pharmacy Ooty-643001 India. 3Department of pharmaceutical Bio technology, Nandha College of pharmacy, Erode, India.
ABSTRACT A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Efavirenz from pharmaceutical formulation. The method was carried out on a Princeton SPHER C18 (25 cm x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of acetonitrile: potassium Dihydrogen ortho phosphate (adjusted to pH 3.5 using orthophosphoric acid) (50:50 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 254 nm. Theophylline was used as an internal standard.The retention time of Efavirenz and Theophylline was 2.45 and 3.10 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability.The proposed method can be used for the estimation of Efavirenz in pharmaceutical dosage form. Keywords: Efavirenz, Pharmaceutical Formulation. [Download Article] [Download Certifiate] |