Abstract

ROLE OF CERTAIN AGENTS ON ZINC MODULATION AND INFLAMMATORY PATTERN IN ZINC DEFICIENT DIABETIC RATS

Mohamed Mahmoud Elseweidy*, Nabila Zein, Nada Mohamed and
Essam Abd Elghani

ABSTRACT

Aims and Objectives: to configure the pattern of inflammation and diabetic state in alloxan diabetic rats fed on zinc deficient diet. Secondly to illustrate the effect of two selected agents, namely zinc gluconate and omega-3 fatty acid (fish oil) on modulation for diabetic state and zinc state. Materials and methods: diabetes was induced by intraperitonially administration of alloxan monohydrate (120 mg /kg) body weight; zinc deficient state was done by feeding zinc deficient diet and deionized water to rats for 28 days and monitor of serum zinc level at interval times. Thirty male albino rats were divided into 5 groups; first one was fed on normal based diet, referred to normal group. Second group is alloxan diabetic rats, received no treatment and referred to diabetic control (DC). Group 3, diabetic rats received zinc deficient diet and referred to as positive control. Group 4 diabetic and zinc deficient rats, received fish oil 50 mg/kg body weight daily for 8 weeks while group 5, diabetic zinc deficient rats, received zinc gluconate 150 mg/kg body weight daily for 8 weeks. Results: Our report showed that treatment with zinc gluconate and fish oil significantly reduced serum glucose, inflammatory markers CRP, TNF-α, IL-6, IL-1β, and IFNγ along with an increase in serum zinc and pancreatic ZNT8 and IL-1β.

Keywords: Type1 diabetes mellitus; inflammatory markers; zinc deficiency; Fish oil; zinc gluconate; zinc transporter 8 (Znt8).