Abstract

PHYSIOLOGICAL IMPACTS OF CHITOSAN SINGLY OR IN CONJUGATION WITH H2 RECEPTOR ANTAGONIST DRUG DURING NSAID THERAPY IN RATS

Nashwah I. Zaki, Lobna A. Hassanin, Amany Hegab, *Mehrevan Abd El- Moniem

ABSTRACT

Aspirin is a potent nonsteroidal anti-inflammatory drug that is used for the treatment of rheumatoid arthritis and related diseases as well as the prevention of cardiovascular thrombotic diseases. The aim of the present study is to explore how chitosan alleviates gastric inflammation induced by aspirin in rats and investigate whether chitosan singly or in-conjugated with Ranitidine could be more effective. Fifty four healthy female rat, were divided into nine groups. Aspirin by two doses (7 or 27 mg/ kg) combined administration with ranitidine (27 mg/kg) as reference drug or chitosan 2% or conjugate of chitosan with ranitidine by the same concentration via oral rout for ten days. Chitosan singly produced significant effects on gastric acidity,ulcer index, mucosal inetrlukin-1β, tumor necrosis factor-α, nitric oxide, myeloperoxidase, protein thiols (P<0.05) and some serum parameters against aspirin treatments. As well as, conjugated treatments showed the highest degrees of normalization against aspirin or ranitidine treatments in most tested parameters. So, the current study give the evidence that chitosan could alleviate gastric inflammation in rats and strongly suggested to be a potential antiulcer drug equivalent to ranitidine. Collectively, Therapy with conjugation of chitosan and ranitidine is the better choice than monotherapy with Ranitidine or chitosan against the selected doses of aspirin.

Keywords: Chitosan – Ranitidine- gastroprotective parameters-immunomodulatory cytokines-mucosal myeloperoxidase.