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SIMULTANEOUS DETERMINATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY THIN-LAYER CHROMATOGRAPHY-DENSITOMETRIC METHOD
Dobrina D. Tsvetkova*, Danka P. Obreshkova, Valentina B. Petkova,
ABSTRACT
The aim of current study was to validate a thin-layer chromatographydensitometric method for simultaneous determination of Valsartan and thiazide diuretic Hydrochlorothiazide in tablets. TLC-densitometric method with system: Camag TLC densitometer; stationary phase: precoated with Silicagel G60F254 plates, 10 mm x 20 mm; mobile phase: chloroform: methanol : toluene : acetic acid = 30 : 10 : 5 : 0.5 v/v; detection at λ = 262 nm was applied. The content of Valsartan and Hydrochlorothizide in tablets was obtained by method of reference standard. For all results Chauvenet's criterion is lower than standard requirements: U St. < 1.73 (N = 6). Accuracy is presented by the degree of recovery R [%], which suit relevant confidence interval: Valsartan: 93.51 %  100.19 %; Hydrochlorothizide: 85.57 %  111.21 %. The content of compounds in tablets correspond to the relevant confidence interval: Valsartan: 149.60 mg  160.32 mg; Hydrochlorothizide: 10.69 mg  13.91 mg. The applied method is appropriate for an estimation of Valsartan and HCTZ in fixed combination in tablets for treatment of hypertension. Keywords: Valsartan, Hydrochlorthiazide, hypertension, thin-layer chromatography, densitometry. [Download Article] [Download Certifiate] |