Abstract

HPTLC METHOD FOR QUANTIFICATION OF GARCINOL FROM DRY FRUIT RINDS OF GARCINIA INDICA AND ITS MARKET FORMULATION.

Ankit M. Patel, Sindhu B. Ezhava*, Ishwarsinh S. Rathod, Mahesh T. Chhabria, Arpit H.Patwari

ABSTRACT

Garcinol, polyisoprenylated benzophenone, a natural histone acetyltransferase inhibitor, extracted from dried fruit rinds of Garcinia indica. Garcinia indica is well spread in tropical rain forest of Western Ghats of India. High performance Thin Layer Chromatographic (HPTLC) method was developed for the estimation of Garcinol from dried fruit rinds of Garcinia indica and its marketed formulations. Chromatography was performed on silica gel G 60F254 as stationary phase and the mobile phase optimized was Toluene: Ethyl acetate: Formic acid (4: 1: 0.5 v/v/v) with pre-saturation time of 30 minutes. The linearity was found to be within range 200-700 ng/spot with mean % recovery of 101.12 ±1.338 %.The proposed method was successfully applied for estimation of Garcinol in fruit rinds of Garcinia indica and the market formulations of Garcinia indica (Trayodashang Guggul by Pathanjali Ayurvedic Ltd. and slimmarz capsules by Medisys Biotech Pvt. Ltd). The amount of Garcinol was found to be 2.5, 0.701 and 0.760 %w/w in Garcinia indica fruit rinds, Trayodashang Guggul and slimmarz capsules respectively.

Keywords: Garcinol, Garcinia indica, HPTLC METHOD.