Abstract

ESTIMATION OF PYRIDOXINE HYDROCHLORIDE IN PHARMACEUTICAL ORAL SOLID DOSAGE FORM BY UV-SPECTROPHOTOMETRY

V.Niraimathi*, J.Jaisudha and A.Jerad Suresh

ABSTRACT

Four new, simple, sensitive and reproducible spectrophotometric methods have been developed for the estimation of pyridoxine hydrochloride in tablet dosage form. Method A involves the determination pyridoxine hydrochloride by standard absorbance method at 291 nm. Method B and Method C involves the determination of pyridoxine hydrochloride by first derivative spectrophotometry and second derivative spectrophotometry respectively. The normal spectrum was derivatized to first and second order derivative spectrum. The Beer’s concentration was found to be 10-40 μg/mL; Method D involves the determination of Pyridoxine hydrochloride by area under curve.

Keywords: Pyridoxine hydrochloride (VitaminB6); Beer’s law; Standard absorbance method; Derivative spectrophotometry; Area under curve (AUC).