Abstract

PURIFICATION OF TANNASE FROM ASPERGILLUS NIGER UNDER SOLID STATE FERMENTATION

Deepa C Philip*, Lavanya. B, Latha. S

ABSTRACT

Tannin acyl hydrolase (E.C. 3.1.1.20) commonly referred as tannase, is an hydrolytic enzyme that catalyses the hydrolysis of ester bonds present in gallotannins, ellagitannins, complex tannins and gallic acid esters. Tannases are extensively used in food, feed, pharmaceutical, beverage, brewing and chemical industries. Owing to its diverse area of applications, a number of patents have been appeared in the recent past. In the present study Aspergillus niger was investigated for the production of tannase by utilizing wood chips as substrate under solid state fermentation. The purification and molecular mass determination was achieved using DEAE-Sephadex gel filtration chromatography and SDS-PAGE respectively.

Keywords: Tannase, Aspergillus niger, Wood chips, Solid state fermentation