Abstract

ISOLATION AND CHARACTERIZATION OF ANTIBACTERIAL COMPOUND FROM BACTERIA ISOLATED FROM VEGETABLE WASTE DUMPED SOIL

Subbiah Pavithra, Ramasamy Amsaveni*, Muthusamy Sureshkumar and Govindasami Vivekanandhan

ABSTRACT

Nowadays, the widespread application of antibiotics results in resistant microorganisms all over the world. Thus screening for the products with antimicrobial activity have been lead to probe natural sources of antimicrobial agents to find new pharmaceutical products. In this view the present research was carried out to isolate antibacterial compound producing bacterial isolates from vegetable waste dumped soil site located in Saibaba kovil vegetable market, Coimbatore, Tamilnadu, India. A total of 10 isolates were obtained and all the isolates were subjected to test β-haemolytic and proteolytic activity. About 30% of the isolates possessed both these activities. In the present investigation partial purification of the antibacterial compound showing the activity is a protein which is less than 10kDa. The protein elutes 36-41 has shown the activity against the human pathogens and this is also a confirmation that the protein size is much smaller. Smaller proteins have been proved till date to have more antibacterial activity. The same protein can also be used to check for the presence of other biological activities. This might prove to be a very good pharmaceutical protein. Change in a single aminoacid in many small proteins has proved to show lots of activity as a multi pathogen killing. This protein can also be employed to such tests to commercialize it.

Keywords: Antimicrobial activity, haemolytic activity, proteolytic activity.