Abstract

“SYNTHESIS, CHARACTERIZATION AND EVALUATION OF IN-VIVO ANTI-INFLAMMATORY ACTIVITY OF SOME SYNTHESIZED N-[(3, 5-SUB-4, 5-DIHYDROISOXAZOL-4-YL)METHYL]ANILINE DERIVATIVES”.

Manjunatha E*, Kalpana Divekar, Palaksha M N, Gajanan Sanglikar, Kavitha

ABSTRACT

Chalcones, 1, 3-diphenylpropenones constitute one of the major classes of flavonoids. Chalcones are prepared by condensation between an Aldehyde and Ketone in the presence of sodium hydroxide was used as catalyst, the Isoxazole derivative 3,5-Substituted 4,5-dihydroisoxaozole was prepared by taking 5mmol of Chalcone and 10mmol of hydroxylamine hydrochloride in presence of 12 mmol of Sodium hydroxide as catalyst. N-[(3,5-sub-4,5-dihydroisoxazol-4-yl)methyl]aniline were prepared by substituted Isoxazole derivative (0.1)mol in Methanol, Formaldehyde(0.04mol) and Aniline(0.02mol. The anti-inflammatory activity of the newly synthesized Isoxazole derivatives were carried out using Carrageenan induced rat hind paw edema method. It was observed that compounds N-{[5-(4-methoxyphenyl)-3-phenyl-4,5-dihydroisoxazol-4-yl]methyl}aniline, N- N- {[3-(2-fluorophenyl)-5-phenyl-4,5-dihydroisoxazol-4-yl]methyl}aniline And N-{[5-(2-fluorophenyl)-3-phenyl-4,5-dihydroisoxazol-4-yl]methyl}aniline showed significant activity 38.24, 33.8 and 39.4 %decrease in paw volume respectively at the dose of 50mg/kg body weight comparable with the standard drug Indomethacin 35.29 % decrease in paw volume at the dose 10mg/kg body weight. Other compounds showed moderate and negligible anti-inflammatory activity.

Keywords: Carrageenan, Isoxazole, Chalcones, N-[(3, 5-sub-4,5-dihydroisoxazol-4-yl) methyl] aniline.