Abstract

LOWER DOSES OF VANADATE IN COMBINATION WITH AZADIRACHTA INDICA LEAF EXTRACT RESTORE ALTERED ANTIOXIDANT STATUS IN THE BRAIN OF STREPTOZOTOCIN INDUCED DIABETIC RATS

Jaya Upreti, Shakir Ali, Seemi Farhat Basir*

ABSTRACT

Azadirachta indica leaf extract has been reported to have hypoglycemic effects. Sodium orthovanadate also exerts antidiabetic effects but at relatively high doses with several toxic effects. So, in the present study we investigate the effects of oral administration of Azadirachta indica leaf extract and vanadate, separately and in combination, on the activities of antioxidant enzymes in streptozotocin induced diabetic rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (55mg/kg body wt). Animals were divided into six groups namely non diabetic control, diabetic control, diabetic treated with insulin (2U), diabetic treated with leaf extract (500mg/kg body weight),diabetic treated with vanadate (0.6mg/ml) and diabetic treated with both vanadate (0.2mg/ml) and Azadirachta indica leaf extract (500mg/kg body weight). After treatment, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were determine in cytosolic fraction of brain tissue. Treatment with different antidiabetic compounds restored the above enzymes activity to normal. Combined dose of Azadirachta indica and vanadate was found to be the most effective in normalizing these Enzyme activities.

Keywords: Sodium orthovanadate, Azadirachta indica, Streptozotocin diabetes, Oxidative stress, Antioxidant.