Abstract

ANTIBACTERIAL STUDY OF OLIVE-BASED FERULATE ESTERS

Nurul Jannah Abd Rahman, Salina Mat Radzi*, Hanina Mohd Noor and Norlelawati Ariffin

ABSTRACT

Antimicrobial study of synthesized ferulate esters was successfully carried out against both Gram-positive (Bacillus subtilis, Staphylococcus epidermidis and Staphylococcus aureus) and Gram-negative (Salmonella typhimurium, Klabsiella Pneumoniae and Escherichia coli) bacteria. Screening by agar diffusion test was employed for preliminary features followed by Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) assays. Ferulate esters demonstrated anti-bacterial activity with diameter of inhibition zones ranged from 8 to 16 mm for S. typhimurium, E. coli, S. epidermidis and S. aureus. For MIC assay, the minimum concentration of ferulate esters against E. coli was 50% followed by S. typhimurium (33.3%), S. epidermidis (20%) and S. aureus (20%). While for MBC assay, ferulate esters only showed bacteriostatic effect. Therefore, this research proved that the ferulate esters possess moderate antibacterial inhibition against both types of bacteria.

Keywords: Ferulate esters, agar well diffusion, MIC, MBC.