Abstract

SIMPLE AND RAPID ISOCRATIC RP-HPLC METHOD TO DETERMINE LOVASTATIN AND NIACIN IN BULK AND PHARMACEUTICAL FORMULATION

G.Tuljarani*, N.Srinivas, B.Karuna Devi and G.Vimala

ABSTRACT

A simple specific, sensitive, precise and reproducible reverse phase high-performance liquid chromatography (RP-HPLC) method has been developed for simultaneous estimation of lovastatin and niacin. The determination was carried out by using Symmetry C8 (4.6×250 mm, 5μm) column with the mobile phase containing acetonitrile: phosphate buffer (pH4.0±0.5) in the ratio of 65:35 v/v. The optimized flow rate was 0.7 ml/min and the UV detection was carried out at 240 nm. The retention time of lovastatin and niacin were found to be 3.093 min and 6.196 min respectively. The method was found to be linear in the concentration range 2.0 -10 μg/ml and 25-125 μg/ml for lovastatin (LS) and niacin (NC) respectively. The method was validated as per ICH guidelines. The proposed method was successfully applied for the estimation of lovastatin and niacin in pharmaceutical dosage forms.

Keywords: RP-HPLC, Lovastatin, Niacin, Validation.