Abstract

ESTIMATION OF TOTAL PHENOLIC, FLAVONOIDAL CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF MILLINGTONIA HORTENSIS LINN.

Aruna Kumari* and Ram Avatar Sharma

ABSTRACT

The present study deals with the comparison of total phenol, flavonoid and antioxidant activity of different plant parts (leaves, stem, root and flower) of Millingtonia hortensis Linn. methanolic extracts. The highest total phenolic content (63.56 mg GAE/gdw) was observed in stem and lowest phenolic content in flower (27.3 mg GAE/gdw). The highest total flavonoidal content (10.84 mg QE/gdw) was found in stem and lowest in root (0.364 mg QE/gdw). Antioxidant activity of Millingtonia hortensis Linn. was estimated using 1, 1 -diphenyl-2- picrylhydrazyl radical (DPPH). The highest radical scavenging activity was found in stem extracts with the IC50 value of 92.29 µg/ml and the lowest scavenging activity was observed in leaves extracts that was 277.82 µg/m. According to the results of present investigation the plant showed significant antioxidant activity to be used in medicinal preparation for the treatment of various diseases.

Keywords: Millingtonia hortensis Linn., Phenolic content, Flavonoidal content, Antioxidant activity, DPPH (1, 1 -diphenyl-2-picrylhydrazyl radical).