Abstract

SCREENING FOR THE ELITE GENOTYPE OF CALOPHYLLUM INOPHYLLUM L. BASED ON ANTIOXIDANT ACTIVITY AND CALOCOUMARIN-A CONTENT

Steena Roshan Sebastian* and A. John De Britto

ABSTRACT

Calophyllum inophyllum, an associated mangrove species has several medicinal properties and potent antioxidant activity. C. inophyllum collected from different accessions along the east coast Tamilnadu was screened for choosing the elite genotype containing more active compound, thereby showing efficient antioxidant activity. Antioxidant activity was analysed by total flavonoid and phenol content, DPPH and hydrogen peroxide radical scavenging activity and reducing power activity. CalocoumarinA, the active compound present in the methanolic leaf extracts of the samples was quantified by comparing the standard chromatogram of calocoumarinA obtained through High Performance Liquid Chromatography (HPLC). The C. inophyllum belonging to Pitchavaram accession recorded maximum content of calocoumarinA and possessed highest antioxidant activity.

Keywords: Calophyllum inophyllum, flavonoid, phenol, DPPH, hydrogen peroxide, reducing power, HPLC.