Abstract

STANDARDIZATION OF CHOLESTEROL SUSPENSION INDUCED HYPERLIPIDEMIA IN RATS

Swati R. Dhande*, Malvika S. Gursahani, Vilasrao J. Kadam

ABSTRACT

Hyperlipidemia has been ranked as one of the greatest risk factors contributing to the prevalence and severity of coronary heart diseases. An important step in drug screening process is preclinical study and it is important to develop and standardize models for testing of antihyperlipidemic drugs. The dose of cholesterol to induce Hyperlipidemia in rats available in literature varies from 25mg/kg/day to 4g/kg/day and the period of induction varied from 20 days to 60 days. Due to large variations in the dose of cholesterol required to induce Hyperlipidemia available in literature, it was decided to standardize the Cholesterol Suspension induced Hyperlipidemia model. Two doses i.e. low dose and high dose of cholesterol were chosen for standarisation of the model. Low dose was 2.5ml/kg of Suspension of 1% Cholesterol and 0.5% Cholic acid in Coconut oil for 28 days and high dose chosen was 2.5ml/kg of Suspension of 2% Cholesterol and 1% Cholic acid in Coconut oil for 28 days. Parameters such as serum lipid profile, atherogenic indices, fecal cholesterol were evaluated and histopathological study was also carried out. Data obtained was analyzed by One way ANOVA followed by Dunnett’s test and compared with vehicle control. It was found that the high dose of cholesterol i.e. 2.5ml/kg of Suspension of 2% Cholesterol and 1% Cholic acid in Coconut oil for 28 days is effective in inducing Hyperlipidemia and this model can be used further to evaluate the antihyperlipidemic activity of herbs and synthetic drugs.

Keywords: Hyperlipidemia, Cholesterol, Cholic acid, Coconut oil, Serum profile, Atherogenic indices, Histopathological evaluation.