DETOXIFICATION OF AFLATOXIN B1 BY PHYSICAL AND CHEMICAL METHODS
Bedi P. S.* and Agarwal R. K.
ABSTRACT
In present study an attempt has been made to detoxify aflatoxin B 1 in
Basmati rice by physical and chemical methods. Aflatoxin ( AFB 1)
contaminated rice containing 20000-30000 ppbAFB1 were directly
exposed to sunlight for continuously for 5hr in the month of June at
temperature varying from 37°C to 42°C.The results showed that
exposure to direct sunlight reduced the AFB1 content by 10-17%. The
study revealed that exposure of contaminated feed to direct sunlight is
helpful in reducing the AFB1 content. In another experiment Basmati
rice were taken as substrate media were divided in to four groups. First
nontreated ( Control ), propionic acid treated ( PA ), monoprop treated
( MP ) and activated charcoal treated ( AC ). Propionic acid was used @ 0.025, 0.05 & 0.10
µg/g, Monoprop @ 0.50, 1.00 & 2.00 µg/g at the time of innoculation. There was 100%
inhibition of AFB1 production at 0.05 and 0.10 µg/g and 60% at 0.025 µg/g in PA group.
Whereas the maximum concentration of AFB1 was reduced to 50% by monoprop at 2.00
µg/g. However the levels of activated charcoal used were 1.0%, 2.0% & 4.0% for
detoxification of AFB1. The results showed reduction in AFB1 concentration was 40-46%,
75-98% and 72-95% respectively.The order of effectiveness in detoxifying the aflatoxin
contaminated samples was PA > AC > MP.
Keywords: Aflatoxin B1, Aspergillus flavus, detoxification, propionic acid, activated charcoal and sunlight.
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