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DEVELOPMENT AND VALIDATION OF UV SPECTROMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID AND PIPERINE IN HERBAL FORMULATION
Smita G. Patil*, Varsha M. Jadhav, Vilasrao J. Kadam
ABSTRACT A simple, rapid and accurate spectrometric method based on solving simultaneous equation for simultaneous estimation of Gallic acid and piperine was developed. Gallic acid and piperine showed maximum absorbance at 273 and 343 nm respectively, absorbance was measured at these wavelengths for estimation of gallic acid and piperine respectively. Gallic acid and piperine both obeys beer Lambert's law in the concentration range of 2-12µg/ml. method was developed and validated as per ICH guidelines and can be applied for the routine estimation of the gallic acid and piperine in herbal formulation. Keywords: Gallic acid, Piperine, Simultaneous equation, UV spectrophotometer, Validation. [Download Article] [Download Certifiate] |