Abstract

OPTIMIZATION OF VARIOUS PARAMETERS FOR PRODUCTION OF ANTIMICROBIAL COMPOUNDS BY FUSARIUM ROSEUM FGCC#61

Shyamji Shukla*, Harshita Shukla and Akhilesh Kumar Pandey

ABSTRACT

The increasing side effects of chemical antimicrobial compounds and growing resistance against them in pathogenic microorganisms has attracted the attention of researchers to search for some novel antimicrobial compounds from natural sources. The most common sources are bacteria, fungi and plants. But the production of these compounds is largely affected by certain parameters like pH, temperature, media constituents etc. Adjustment of these parameters up to optimum level leads to maximum production of antimicrobial compounds. Therefore the aim of present study was to investigate the influence of cultural conditions and environmental parameters affecting the growth and antimicrobial metabolite production of the Phytopathogenic fungi Fusarium roseum FGCC#61 isolated from Vinca rosea. The effect of different culture media, pH, temperature, incubation period, various carbon and nitrogen sources on the growth and antimicrobial metabolite production in a fixed volume of culture broth were studied. Fusarium roseum FGCC#61 grew well and produced optimum antimicrobial metabolites in Richard’s broth medium at 25±2°C on the 21 st day of the incubation. The optimal growth, however, was obtained at pH 6. Sucrose and Sodium nitrate were the best carbon and nitrogen sources respectively for optimum growth and production of active metabolites by the isolate. The results of this investigation indicated that cultural conditions like carbon, nitrogen sources as well as physical factors such as temperature, pH and incubation period greatly affected the growth and production of antimicrobial metabolites by Fusarium roseum FGCC#61.

Keywords: Optimization, Phytopathogenic fungi, Antimicrobial compound.