Abstract

PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ANNONA RETICULATA RAW FRUIT PEEL EXTRACTS

DSVGK Kaladhar*, K Apparao Rayavarapu

ABSTRACT

The present study describes the phytochemical profile, antioxidant and antimicrobial activities of Annona reticulata raw fruit peel extracts collected in Appannapalem, Jami mandal. Vizianagaram, India. The study aimed to evaluate the phytochemical analysis, antioxidant and antimicrobial activities of methanol, ethanol, ethyl acetate and aqueous raw fruit peel extracts of Annona reticulata. Phytochemical studies with Annona reticulata raw fruit peel extracts showed that all tested extracts contains alkaloids, cholesterol, coumarins, flavonoids, phenols and saponins. In addition steroids are present in ethyl acetate and terpenoids are present in aqueous extracts. The antioxidant results significantly shown good IC50 value with methanol and ethanol extracts respectively (148 μg/ml). The standard compound (ascorbic acid) has shown IC50 value as 120 μg/ml. The antimicrobial test results showed that plant raw fruit peel extract had a great potential antimicrobial activity against all 6 bacteria and 2 fungal strains. The antibacterial activity of all the four extracts of Annona reticulata plant fruit peel was determined by agar well diffusion method. The well size with 6mm at four different concentrations i.e., 10μl, 25μl, 50μl, 100μl were tested. The screening results showed that highest inhibition was observed by the methanol extract followed by ethanol, ethyl acetate and aqueous extracts for Annona reticulata raw fruit peel. Maximum activity was observed at highest concentration (100μg/ml). The results presented here may suggest that the raw fruit peel extracts of Annona reticulata possesses antioxidant and antimicrobial properties, and is therefore a potential source of ingredients for the food and pharmaceutical industry.

Keywords: Antimicrobial activity, Phytochemical analysis, Antioxidant activity, Annona reticulata.