Abstract

SYNTHESIS AND ANTIMICROBIAL ACTIVITIES OF 2-(4-SUBSTITUTEDPHENYL)-1,10B DIHYDROSPIRO [BENZO [E] PYRAZOLO [1, 5-C] [1, 3] OXAZINE-5, 3’-INDOLIN]-2'-ONE DERIVATIVE FROM ISATIN

Mayuri A. Borad, Manoj N. Bhoi, Dhanji P. Rajani, Smita D. Rajani, Hitesh D. Patel*

ABSTRACT

This research article mainly emphases on recent endeavours in the field of spiro heterocyclic compounds.2-(4-substitutedphenyl)-1,10bdihydrospiro [benzo[E] pyrazolo[1,5-C] [1,3] oxazine-5,3’-indolin]-2'-one derivative were synthesized by the sequential reaction of o-hydroxy benzaldehyde and various 4-substituted acetophenones and a mixture of isatin and 2-(3-(4-substitutedphenyl)-4, 5-dihydro-1H-pyrazol-5-yl) phenol in the presence of PTSA. All the spiro compounds were prepared via microwave assisted reaction and obtained in good yield as compared to conventional method. All compounds were characterized by IR, 1H NMR, 13C NMR, Mass analysis and elemental analysis. All the synthesized compounds were evaluated for antibacterial and antifungal activities. Some of the synthesized compounds showed good antibacterial and antifungal activities with 50 and 250 μg/mL MIC (minimum inhibitory concentration) respectively.

Keywords: Benzopyrazolo-oxazineindoline derivative, Chalcones, Isatin, Pyrazole Derivative, P-Toluene Sulphonic Acid (PTSA), Spiro Heterocyclic Compound.