Abstract

CHARACTERIZATION AND EVALUATION OF ANTIBACTERIAL ACTION OF BIOSYNTHESISED ZINC OXIDE NANOPARTICLES USING TINOSPORA CORDIFOLIA LEAF EXTRACT

Priyanka Choudhary, Dr. Seema Parveen and Dr. Rajni Bais*

ABSTRACT

The biosynthesis and antibacterial activity of ZnOTC-NPs against Escherichia coli, a Gram-ve bacillus, are the main subjects of this work. To do this, leaf extract from the herbaceous vine Tinospora cordifolia (TC), which is native to tropical regions of India, was used to biosynthesize zinc oxide nanoparticles (ZnO-NPs). This T. cordifolia leaf extract is employed in Ayurvedic medicine and serves as a reducing and stabilizing agent during the production of ZnO-NPs. The analytical methods employed to characterize ZnOTC-NPs included DLS (average diameter of ZnOTC-NPs was 74.04 nm), FT-IR (confirmed the presence of functional groups involved in stabilization, reduction, and capping of ZnOTC-NPs), and UV-Vis spectroscopy (absorption band for ZnOTC-NPs was observed at 355 nm). With the formation of inhibition zones against Escherichia coli (20.8 mm at 100% concentration dosages) and an IC50 value of 4.067μg/mL, these ZnOTC-NPs demonstrated dynamic antibacterial activity. According to these results, ZnOTC-NPs may have antibacterial properties. After intensive in vivo research concentrating on cellular level investigations, ZnOTC-NPs reinforced with T. cordifolia extract thus have the potential to be employed as an antibacterial agent and can be used as an alternative to commercially available medications.

Keywords: Tinospora cordifolia, biosynthesis, zinc oxide nanoparticles (ZnOTC-NPs), antibacterial activity.