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APOPTOTIC ACTIVITY OF ETHANOLIC EXTRACT OF MORINGA OLEIFERA ROOT BARK ON HUMAN MYELOID LEUKEMIA CELLS VIA ACTIVATION OF CASPASE CASCADE
Subhadeep Roy, Nilanjana Deb, Santanu Basu, Shila Elizabeth Besra*
ABSTRACT
Identification of cytotoxic compounds that induce apoptosis has been the mainstay of anti-cancer therapeutics. The plant Moringa oleifera Lam. (family: Moringaceae) is widely used in Indian traditional medicine as a broad spectrum medicine. The ethanolic extract of Moringa oleifera root bark (EMORB) and its fraction F1 & F2 showed significant anti-leukemic activity in human leukemic cell lines with EMORB- IC50 of 30.22μg/ ml, and 28.29μg/ml, F1-9.73μg/ ml, and 10.23μg/ml and F2-8.56μg/ ml, and 9.12μg/ml in U937 & K562 cell lines respectively. EMORB treated leukemic cells showed chromatin condensation, apoptotic body formation and increased Caspase 9 and 3 production indicating apoptosis. Confocal microscopy images showed externalization of phosphatidylserine from inner leaflet to outer leaflet of the cell membrane in EMORB treated both the human myeloid leukemic cell lines. Flow Cytometric analysis showed appreciable Keywords: Moringa oleifera, Root Bark, Myeloid, Apoptosis, Caspase. [Download Article] [Download Certifiate] |