Abstract

EXTRACTION OF ACETYLCHOLINE ESTERASE INHIBITORS FROM JACARANDA ACUTIFOLIA AND SCREENING OF ITS ACTIVITY ON ZEBRAFISH BRAIN.

Shreyas Meda, Praveen A, Suvratha J, Anmol Kulkarni, Guruprasad R*

ABSTRACT

Acetylcholinesterase (AChE) is an enzyme involved in the breakdown of the neurotransmitter acetylcholine into acetic acid and choline. Inhibition of Acetylcholinesterase is needed for deceleration of neurodegeneration in Alzheimer’s, Parkinson’s and other neurodegenerative diseases. Plants are known to contain Acetylcholinesterase inhibitors. The methanolic extracts from leaves of Jacaranda acutifoliawere checked for Acetylcholinesterase inhibition on the acetylcholinesterase from brain homogenate of Danio rerio. We checked the activity of the enzyme in in-vitro and in-vivo conditions. In our investigations the extract from the plant showed a reduction in the AChE activity.Under in-vitro conditions and in-vivo conditions the activity of AChE dropped by 15.07% and 38.93% respectively.

Keywords: Alzheimer’s Disease, Acetylcholinesterase, Inhibition, Zebrafish,Plant Extract.