Abstract

A VALIDATED LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF SULFADOXINE AND PYRIMETHAMINE IN PHARMACEUTICAL FORMULATIONS

M. Kusuma Kumari, Jyothi K Kasthuri, B. Hari babu*, P.V.V. Satyanarayana

ABSTRACT

An accurate, simple and precise RP-HPLC method for the simultaneous determination of Sulfadoxine and Pyrimethamine in pharmaceutical formulations was developed and validated. Chromatographic separation of two drugs was achieved on a Kromasil C18, (250x4.6nm, 5μm) column by using the mobile phase consisted of methanol, acetonitrile and 0.1% ortho phosphoric acid in the ratio 10:50:40(v/v) at a flow rate of 1mL/min and the wavelength of detection was at 223 nm. The retention time for Sulfadoxine and Pyrimethamine were found to be 4.67 and 6.74 min respectively. The linearity of the developed method was tested over a concentration range of 50-350 μg/mL for Sulfadoxine and 2-16μg/mL for Pyrimethamine and the correlation coefficient (r2) of regression for both the drugs was 0.999 which is almost equal to 1. The limit of quantification was 16μg/mL for Sulfadoxine and 0.8μg/mL for Pyrimethamine and the limit of detection was 5μg/mL for Sulfadoxine and 0.25μg/mL for Pyrimethamine. The mean percentage recoveries were 99.15% for Sulfadoxine and 99.3% for Pyrimethamine respectively.

Keywords: Sulfadoxine, Pyrimethamine, RP-HPLC, Pharmaceutical formulation, Simultaneous estimation.