Abstract

AMELIORATION OF RADIATION MEDIATED BIOCHEMICAL ALTERATIONS IN KIDNEYS OF MICE BY TINOSPORA CORDIFOLIA ROOT EXTRACT

Priyanka Sharma*, Jyoti Parmar, Priyanka Sharma, Preeti Verma, P.K.Goyal

ABSTRACT

The present study was carried out to investigate the radiation induced biochemical alterations in kidney of mice and their possible amelioration by radioprotective effect of Tinospora cordifolia root extract (TCE). For this purpose, adult male Swiss albino mice were divided into four groups. Group I (Sham irradiated), Group II (TCE administratered), Group III (5 Gy Irradiated control) and IV (Irradiated experimental). Animals from all the above groups were autopsied after 12 hrs, 24 hrs, 3rd day, 7th day, 15th day and 30th day of treatment. The kidney was taken out and different biochemical parameters such as total proteins, cholesterol, glycogen, lipid per oxidation (LPO), glutathione (GSH) and calatase activity were estimated. Irradiation resulted in a significant decrease in protein, cholesterol, GSH and catalase but an increase in glycogen and lipid per oxidation (LPO) as compared to normal. Conversely, TCE pretreatment reduced the radiation- induced alterations in all such parameters and the recovery was faster as compared to irradiated control. TCE pretreatment resulted in a significant increase in the proteins, cholesterol, GSH and catalase; whereas, glycogen and LPO showed a significant decrease from the respective irradiated control group at the all the autopsy intervals. Hence, the results from the present study suggest that TCE pretreatment provides protection against radiation- induced biochemical dysfunction in kidney.

Keywords: Gamma radiation, Kidney, LPO, GSH, Catlase, Tinospora cordifolia, Swiss albino mice.