Abstract

HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY FINGERPRINTING OF PHYTOSTEROLS FROM CASSIA OCCIDENTALIS LEAVES

Mudit Kumar*, Amita Tilak and Sudhir Singh Gangwar

ABSTRACT

Objective: Natural remedies from medicinal plants are found to be safe and effective. Many plant species have been used in folklore medicine to treat various ailments. Standardisation of plant materials is the need of the day To study flavonoid profile of the medicinal plant. Cassia occidentalis (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: The extracts were tested to determine the presence of various phytochmeicals like alkaloids, phenolic compounds, phytosterols, carbohydrates, glycosides, saponins, terpenoids, tannins, fixed oils, fats and protein and aminoacids (Harborne and Harborne, 1998). HPTLC studies were carried out by Harborne and Wagner et al method. Different compositions of the mobile phase for HPTLC analysis were tested in order to obtain highresolution and reproducible peaks. CAMAGHPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of phytosterols of hydroalcohol extract of leaves revealed nine polyvalent phytoconstituents (09 peaks) and corresponding ascending order of Rf values in the range of 0.010 to 0.826. Conclusion: With the results of HPTLC analysis and Rf values Phytosterols have been concluded in the extract. Hence it was concluded that the phytosterol compounds present in the Hydroalcohol extract could be responsible for antioxidant activities. Plant derived antioxidants, especially phenols and phytosterols, have been described to have various properties like anticancer, antiaging and prevention of cardiovascular diseases. Furthur, separation and characterization of the bioactive compound from the plant is to be evaluated and reported in near future.

Keywords: HPTLC, Cassia occidentalis leaves, Hydroalcohol extract, Phytochemicals, Phytosterols, Fingerprinting.