Abstract

PURIFICATION (CRYSTALLIZATION) OF BIOACTIVE INGREDIENT ANDGROGRAPHOLIDE FROM ANDROGRAPHIS PANICULATA

Dilip Jadhao* and Bhaskar Thorat

ABSTRACT

In the study, the purification of Andrographolide from the Andrographis Paniculata was carried out using different physical separation techniques such as extraction and crystallization followed by drying. The extraction of the andrographolide from the A. Paniculta was carried out using different solvents. The effect of andrographolide to solvent ratio on extraction efficiency was studied. It was found that the andrographolide to solvent ratio of 1:3.5 w/v gives higher percentage purity of andrographolide. The solubility study of andrographolide was studied in order to find out the best solvent for crytsallization. Subsequently, the extract which was obtained after extraction was treated with activated charcoal to get rid of the undesired impurity which may hinder the process of crystallization. Subsequent to extract clarification, extract was concentrated by evaporation. The process of cooling crystallization was effectively employed for further purification of andrographolide with the recovery of 95% andrographolide with high purity. The process of crystallization was studied in terms of supersaturation (more refined product). The andrographolide was confirmed by LCMS and Melting point. Scanning electron and inverted microscopy were applied to find out the morphology and the size of purified andrographolide. It was observed that andrographolide gives different size of cube shaped whitish crystals in the range to 30μm- 40 μm.

Keywords: Crystallization, herbal products, medicinal plant, anti-pyretic