Abstract

ENHANCING THE BIOAVAILABILITY AND PERMEABILITY OF RESORCINOL ORNAMENTED TRANSFEROSOME TRANSDERMAL PATCH FOR DUAL THERAPY: PRODUCTION, EVALUATION, AND CELLULAR UPTAKE INVESTIGATION USING L-6 AND A143

Sandhiya*, Masilamani K., Deepa, Salma, Rajavignesh, Santhanalakshmi, Sharon, Shanmuga Sundaram

ABSTRACT

Purpose: Examining a tranfersome formulation's potential for transdermal delivery of Resorcinol was the aim of the current investigation. Resorcinol is a potent herbal remedy having anti-inflammatory, anti-diabetic, and anti-cancer effects that is widely used. has a low oral bioavailability despite having a high pharmacological activity. To circumvent this, nano carriers may increase the drug's penetration and bioavailability. Methods: Additionally, a thin film evaporation method was used to create the transfersomes, solvent casting was used to create the patch, and the Box-Behnken design was used to optimize the finished product. This design was used to optimize the Resorcinol loading by considering the effects of three factors (X1: lipid, X2: polymer, and X3: drug) on the response variables Y1 (EE), Y 2 (size), and Y 3 (drug release). transfersomal patch. Different concentrations of surfactants, such as Tween 80 andSpan 80, were utilized to construct the transfersomal patch. SEM, TEM, and other methods were used to characterize the generated transfersomes. Result and Discussion: When compared to span 80, the in-vitro release study showed 92% drug release using tween 80 surfactant, and the DPPH assay activity produced notable results when compared to the ascorbic acid standard. As the glucose tolerance assay and MTT assay showed, the generated transfersomal patch had more potent inhibitory efficacy than traditional medications. The entrapment efficiency was shown to be influenced by the PC (Lecithin): Edge Activator (Tween 80 & Span 80) ratio. A greater entrapment of 89.6±0.049 was seen inside the TW4 formulation. The average vesicle size for formulations SP2 and TW4 was found to be between 100 and 250 nm, and this value was also related to the formulation's entrapment efficiency. Permeability was also impacted by the PC (lecithin) to Edge Activator (Tween 80 & Span 80) ratio. The fact that the formulation TW4, which showed increased entrapment efficiency, provides higher drug permeation from transfersomal patch, supports this. The results of this study suggest that PC: TW 80 transfersomes could be a helpful way to gradually increase the permeability of Resorcinol. Conclusion: From the aforementioned findings, it was determined that, although there was not yet a herbal transdermal patch for diabetics, additional therapeutical evidence was required to support the introduction of such an effective plant-based product into the market. In the future, it would be a very promising therapy for dual therapy.

Keywords: Resorcinol, optimization study, L-6 cell line, A143 Cell line, DPPH assay.