Abstract

AN INVITRO STUDY OF POLYHERBAL FORMULATION

S. Rajini*, M. Prasad Rao, M. David Prudhvi Raj, G. Pujita, M. Tejaswini and T. Sailaja

ABSTRACT

This study presents an in vitro investigation of a polyherbal formulation, aiming to explore its pharmacological properties and potential therapeutic applications. The formulation, prepared from a blend of traditional medicinal herbs, was subjected to various assays to assess its antioxidant, anti-inflammatory, antimicrobial, and cytotoxic activities. Results indicate significant antioxidant potential, dose-dependent inhibition of pro-inflammatory markers, potent antimicrobial efficacy against selected microorganisms, and selective cytotoxicity towards cancer cell lines. These findings highlight the multifaceted pharmacological profile of the polyherbal formulation, suggesting its promising role in healthcare. Further research is warranted to elucidate its mechanisms of action and evaluate its
therapeutic efficacy in vivo. Background: The use of herbal plant extracts in wound healing is known through decades, but it is necessary to provide scientific data through reverse pharmacology. Objective: The aim of the present study is to find the mechanism behind the healing of wounds using invitro and in vivo assays. Material and Methods: The study was designed to determine proliferation and mobilization of fibroblast and keratinocytes at the site of injury, angiogenesis at the site of healing and reduction in oxidative stress while healing. In our earlier studies it was observed that herbal extract of Vitex negundo L. (VN), Embolic officinalis Gaertn (EO), and Tridax procumbens L. (TP) showed rapid regeneration of skin, wound contraction, and collagen synthesis at the site of injury in excision wound model. In the present study the cell mobilization was monitored in the scratch assay on L929 fibroblastic cell line and HaCaT keratinocytes cell line under the influence of aqueous plant extracts and its formulation. This formulation was also assessed for its angiogenic potential using CAM assay. Study was carried out to probe synergistic effect of polyherbal formulation using excision model in rat.

Keywords: in vitro study, polyherbal formulation, traditional medicine, pharmacological properties, antioxidant activity, anti-inflammatory activity, antimicrobial activity, cytotoxicity, herbal medicine, synergistic effects.