Abstract

ANTIMICROBIAL AND ANTIOXIDANT PROTEINS FROM THE CRAB LIAGORE RUBROMACULATA (DE HAAN, 1835)

E. Rethna Priya*, S. Ravichandran and P. Jawaharlal

ABSTRACT

The emergence of new infectious diseases and resistance to the antibiotics by the existing ones led to the new sources for drug discovery. In the present study antibacterial and antioxidant activity of the hemolymph of brachyuran crabs Liagore rubromaculata was investigated. In the antibacterial activity against 6 pathogenic strains maximum zone of inhibition was recorded against highest zone of inhibition was observed in the hemolymph of female crab L. rubromaculata against P. vulgaris(12 ± 1 mm) and the minimum activity was observed against E.coli(6 ± 1 mm). In the hemolymph of male crab L.rubromaculata the highest zone of inhibition was observed Against E. faecalis(9 ± 1 mm) and the minimum activity observed against K. oxytoca (7mm). In TLC appearance of pink spots indicates the presence of protein in the tested sample. In SDS PAGE, in male four bands were detected in the gel that represents the presence of proteins in the range nearly 26.5-73 kDa. In female five bands were detected in the gel that represents the presence of proteins in the range nearly 25.2-75 k Da. Hence the present study indicated that the hemolymph of L. rubromaculata crabs may potential antibiotics.

Keywords: Antimicrobial proteins, DPPH, Hemolymph, TLC, SDS.