Abstract

APPLICATION OF HPLC IN PHARMACEUTICAL ANALYSIS: AN OVERVIEW

Rohit S. Kshirsagar*, Rameshwar P. Tathe, Prashant S. Malpure and Rishikesh S. Bacchav

ABSTRACT

Overview of chromatography in general Chromatography, derived from the Greek words chroma, which means "color," and graph, which means "to write," refers to a group of methods used in laboratories to separate mixtures. n contemporary analytical chemistry, one of the most adaptable and popular methods is high performance liquid chromatography (HPLC). Since its invention in the 1960s, high-performance liquid chromatography (HPLC) has developed into a standard technique for the identification, quantification, and separation of a wide range of substances in a variety of scientific fields and enterprises. High performance liquid chromatography has become a classic, reliable, and powerful analytical technology in pharmaceutical analysis and is widely used due to its strong specificity, high sensitivity, fast speed, high resolution, easy operation, and wide
application range. It demonstrates the ability to separate and quantify active ingredients, additives, degradation and impurity compounds, and different solid dosage forms of target drugs. HPLC is based on the principles of chromatography and utilizes the differential interactions between analytes in the mobile phase of a flowing liquid and the stationary phase to achieve separation. The quantification of active pharmaceutical ingredients (APIs) and associated compounds is one of the main applications of HPLC in pharmaceutical studies. Pharmaceutical experts can monitor medication content homogeneity, evaluate batch-to-batch consistency, and identify the presence of contaminants or degradation products thanks to the precise and accurate drug concentration measurements that HPLC could compromise medication efficacy or raise safety issues.

Keywords: HPLC, Pharmaceutical Analysis, Active Pharmaceutical Ingredient (API), Validation.