Abstract

HISTOCHEMICAL CHANGES IN CONCURRENCE TO TEMPERATURE FLUCTUATIONS PERTINENT TO ACID PHOSPHATASE STAIN IN LYMNAEA (RADIX) LUTEOLA

Trijit Nanda*, Sandipan Mondal and Malabika Bhattacharjee

ABSTRACT

Histochemical methods are often adopted to ascribe the biochemical characteristics of the neurosecretory materials in both vertebrates and invertebrates. This has a bearing with respect to conventionally stained materials and the secretory contents. Series of histochemical tests for neurosecretory materials in the divergent groups of invertebrates including pulmonates have revealed that the neurosecretory cells (NSCS) have three principal constituents -Proteinaceous, glucidic and lipidic in different proportions and accordingly in some cases the neurosecretory materials may be considered glycoprotein, lipoprotein and glycolypoprotein. 

In the present investigation the cytochemical test for the localization of Acid Phosphomnoesterase in the neurosecretory materials (NSM) are well documented in the different temperature regimes—Low (4-100 C), Near ambient (20-250 C) and (High 30-350 C). Moreover, this has been ascertained that Acid phosphatse activity is mainly due to presence of lysosomes and formation of autophagic vacuoles.

Keywords: Histophysiological, Neurosecretory cells, Acid phosphatase, Histochemical analyses, Cerebral neurosecretory elements.