Abstract

PROTECTIVE EFFECTS OF LIPPIA CITRIODORA ON ACETIC ACID INDUCED ULCERATIVE COLITIS IN RATS

Roya Hassan pour, *Mirzaei Ali

ABSTRACT

Object and aim: ulcerative colitis (UC) is a chronic inflammation of the intestinal tract disease in which immune response was suppressed due to high levels of free radicals and inflammatory agents. The aim of the present study was to evaluate protective effect of Lippia citriodora on ulcerative colitis by different biochemical tests. Methods: Rats were randomized into four groups each containing eight rats. Rats were administered 500 mg/kg Lippia citriodora once daily for four days before ulcerative colitis induction by 4% acetic acid (AA) and three days after AA rectal administration. animals were scarified and the colonic tissues were dissected. In colon tissues, levels of glutathione (GSH), malondialdehyde (MDA) , nitric oxide (No) and interleukin-1β (IL-1β were estimated. Results: Acetic acid administration obviously reduced GSH, while MDA, IL-1β, and NO levels were increased in colon of rats with UC compared controls. Administration of Lippia citriodora extract ameliorated the biochemical alterations due to acetic acid induced UC, by decreasing malondialdehyde, interleukin- 1 β , nitric oxide and increasing reduced glutathione level, in colon tissue. Conclusion: This study concluded that use of Lippia citriodora extract could be helpful in preventing the relapse of UC disease due to their possible antioxidant and anti-inflammatory effects.

Keywords: Interleukin - 1?, Lippia citriodora, ulcerative colitis, oxidative stress markers.