Abstract

ANALYSIS TOTAL FLAVONOID CALCULATED AS GENISTEIN, ANTIOXIDANT ACTIVITY AND ANTI-INFLAMMATORY PROPERTIES OF CRANBERRY PLANT ETHANOL EXTRACT

Resmi Mustarichie*, Moelyono Moektiwardoyo, Yoga Windu Wardhana,
Danni Ramdhani

ABSTRACT

Raw cranberries and cranberry fruit are abundant food sources of flavonoids such as proanthocyanidins, flavonols and quersetin. Reviews These compounds have demonstrated activity as a possible anti-cancer agents in vitro. In previous research has been done proving the interaction of test compounds quersetin computing that is nutritious content of the stems and leaves of cranberries with an anti-inflammatory receptor H4R. Cytotoxicity properties of cranberries for this plant..This study reports on the phytochemical screening, total flavonoids calculated as quercetin, antioxidant activity as well as anti-inflammatory of ethanol extract of cranberry. Antioxidant activity was carried out by DPPH method. Testing antiinflammatory activity performed by the method of induction of carrageenan and total flavonoids calculated as quercetin done by spectrophotometry. It was found that the herbs that we checked positive compounds containing alkaloids, flavonoids, tannins and polifenolat, triterpenoids and steroids, as well as monoterpenoid and sesquiterpenoid quinones, and negative for saponins. From the results of this experiment, the percentage content of total flavonoids in cranberry herbal at 0.099% w/w. IC50 of antioxidant activity of cranberry ethanol extract was found lower than its vitamin C as control positive. The antioxidant activy might be due to flavonoid content.The test results indicated that the anti-inflammatory activity of extracts of cranberry plants had inflammation inhibition percentage of 36.47%, 40.07% and 51.27%. It was found that extracts of a dose of 1000 mg/kg was the most good in the inhibition of inflammation. The results of this study support previous research that the cranberry extract can be used as an adjuvant therapy in the treatment of inflammation the caused by cancer.

Keywords: Cranberry, anti-inflammatory activity, quercetin, antioxidant, cancer.