Abstract

ANTIOXIDANT ACTIVITIES OF IRANIAN BITTER VETCH IN DIFFERENT METHODS

Ali Mirzaei*,Mohammad Satarifar, Farideh Badakhshan Zadeh

ABSTRACT

Introduction: Bitter vetch is an ancient legume crop of the Mediterranean region. It been used mostly for grain, hay production and feed ruminant for increase of milk production. This study was managed for determination of antioxidant activity and phenol contents. Material and Methods: Vicia ervilia (Bitter vetch) aerial plant and seeds were prepared in Yasuj Iran. Extraction was carried out in distilled water with maceration methods. For determination of antioxidant activities used diphenyl-2-picrylhydrazyl (DPPH) ,Trolox equivalent antioxidant capacity (TEAC ) radical scavenging assays and ferric reducing antioxidant power (FRAP) assay for reducing power as an antioxidant potentia. Total phenol and flavonoids were estimated as antioxidant component. Analysis of data was carried out by one way analysis of variance ANOVA. Results: Total phenol and flavonoids content ranged from 62.7-105 mg Gallic acid and 22.1-43 mg Rutin /g samples respectively .The aerial parts had the highest antioxidant activity by DPPH and FRAP methods. The antioxidant activity of the samples in FRAP and DPPH method can be attributed to their total phenolic and flavonoid content. Conclusion: aerial part was reported with high total phenol, flavonoid and antioxidant activity by different systems. The highest antioxidant activity revealed by TEAC assay .it is could be suppose the aerial part of bitter vetch contain both hydrophilic and lipophilic substances with chain-breaking ability of free radicals.

Keywords: Antioxidant Activities, Bitter vetch, DPPH, TEAC.