Abstract

AN OVERALL REVIEW ON ANALYTICAL AND BIOANALYTICAL METHODS DEVELOPED FOR THE ESTIMATION OF GLIMEPRIDE AND METFORMIN

Karthick Rajendran, J. S. K. Nagarajan*, Meiyappan Varadharaj, Avinash Kumar Singh, Jebisel Reba Paul Moses and Lokesh Raja Janarthanan

ABSTRACT

Anti-diabetic medications, such as Glimepiride Metformin, are used to treat type 2 diabetes, particularly in people whose blood sugar levels cannot be maintained by diet and exercise alone. MET and GLM have been formulated as a fixed-dose combination (FDC) and have been commonly employed for the management of type 2 diabetes. This combination has been proven to result in superior glycemic control compared to monotherapy of either drug. This combination in pharmaceuticals can be simultaneously estimated by a number of analytical techniques such as HPLC, UV, Capillary electrophoresis, GC, UPLC, LC-MS/MS. This review summaries available reported analytical methods simultaneous estimation of metformin and glimepiride. The method was validated for linearity, accuracy, precision, detection limit, quantification limit, specificity, system suitability and solution stability. Results of all validation parameters were within the limits as per ICH guidelines. Most of these methods were also validated as per ICH guidelines. The developed method can be used for routine analysis of titled drugs in combined dosage form.

Keywords: Metformin, Glimepiride, biological samples, pharmaceutical formulations-HPLC, UV, Capillary electrophoresis, LC-MS.