Abstract

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY A METHOD OF ANALYS OF DIFFERENT ANTIBIOTIC DRUGS

Gunjan Marathe, Shivani Mahale*, Tejas Magare, Hemant Marathe, Pankaj Mali, Rohit Mistari and Harshjeet Sisode

ABSTRACT

High-performance liquid chromatography (HPLC) has emerged as a powerful analytical technique for the precise quantification and qualitative analysis of various pharmaceutical compounds, including antibiotics. This review article provides a comprehensive overview of HPLC methods developed for the analysis of three widely used antibiotics: penicillin, erythromycin, ceftiaxone. The review encompasses the principles of HPLC, including instrumentation, chromatographic columns, mobile phases, and detection techniques commonly employed in antibiotic analysis. It highlights the significance of HPLC in pharmaceutical research and quality control due to its high sensitivity, specificity, and reproducibility. Furthermore, the article discusses in detail the HPLC methods developed for the analysis of penicillin, erythromycin, ceftiaxone, covering aspects such as sample preparation, chromatographic conditions (Including column type, stationary phase, mobile phase composition, flow rate, and temperature), calibration, and validation parameters. Emphasis is placed on the optimization strategies used to enhance the separation, resolution, and sensitivity of these antibiotics in various pharmaceutical formulations and biological samples. The comparative analysis of different HPLC methods for each antibiotic provides insights into the advancements, challenges, and future prospects in antibiotic analysis using HPLC. The review also discusses the application of HPLC in pharmacokinetic studies, stability testing, and bioequivalence assessments of antibiotic formulations. Overall, this review article serves as a valuable resource for researchers, pharmacists, and pharmaceutical analysts interested in the HPLC analysis of penicillin, erythromycin, ceftiaxone, offering a comprehensive understanding of the analytical techniques and methodologies employed in antibiotic drug analysis.

Keywords: Hplc, Chromatographic condition, Validation, Miantibiotics, Penicillin, erythromycin, Ceftiaxone.