Abstract

EVALUATION OF ANTI ANEMIC ACTIVITY IN ETHANOLIC LEAF EXTRACT OF AMARANTHUS VIRIDIS IN ALBINO WISTAR RATS

Tamilselvan G.*, Avinas M. B. and Senthil Kumar K.L.

ABSTRACT

Background: Amaranthusviridis is an annual herb with an upright, light green stem that grows to about 60-80 cm in height. 3-6 cm long, 2-4 cm wide, with long petioles of about 5cm. It is used in Northeastern Indian state of Manipur, and also eaten as a vegetable in south India especially in kerala. It is traditionally used for the various ailments such as Anti-inflammatory agent of Urinary tract, Anti-rheumatic, Anti-ulcer, Anti-anemic, Laxative, improvement of Appetite, Anti-leprotic, Venereal diseases Vermifuge Diuretic, Treatment of respiratory, Eye problem and Treatment of Asthma. Aim: To evaluate the Anti-anemic activity in Ethanolic leaf extract of Amaranthus Viridis in Albino Wistar Rats. Objective: This study evaluates the hematological and biochemical parameters of Amaranthus Viridis in 2,4 dinitrophenyl hydrazine induced hemolytic anemia in Wistar rats for possible anti-anemic potential. Materials and Methods: This study evaluates the Anti-anemic effect of the leaf extract based on hematological and biochemical parameters and body weight in 2,4-dinitrophenyl hydrazine-induced hemolytic anemia in Wistar rats. Extract were administered into the three groups of rats and the hematological parameters were evaluated. Results: Increase in the level of hemoglobin and PCV of rats fed with raw extracts, followed by rats fed with heated extracts and lastly control rats fed with water and normal feed. There was a progressive increase in total protein level and RBC of rats fed with heated extracts followed by rats fed with raw extracts and lastly control rats. There was a decrease in albumin of rats fed with heated extracts followed by raw extracts while the albumin level of the control rats is increased. Conclusion: The results suggest that the Ethanolic leaf extract of AmaranthusViridis is relatively safe and possesses a significant Anti-anemic property; therefore, it may be a potential lead in the discovery of drug for treatment of hemolytic anemia.

Keywords: Anemia, Anti-anemic activity, Amaranthusviridis, 2,4 dinitrophenyl hydrazine, hemoglobin, protein.