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DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF VILDAGLIPTIN AND DAPAGLIFLOZIN DRUGS IN HUMAN PLASMA BY RP-HPLC
Prathmesh Sudhir Gogate* and Shailesh G. Jawarkar
ABSTRACT
A simple, rapid, precise, and accurate Reverse Phase High Performance Liquid (RP-HPLC) Chromatography method was developed for simultaneous determination of Vildagliptin and Dapagliflozin in Human plasma. The method was performed on a Younglin-HPLC system -SP930D The analyte separation was achieved using an Hypersil BDS C18 (250mm x 4.6mm, Particle size: 5µm) column with a mobile phase consisting of (Methanol: Water: Trifluoroacetic acid, (80:20:0.2) with 1.0 ml/min flow rate. UV detection at 219 nm was employed. The retention time for Vildagliptin and Dapagliflozin was 3.10 min and 3.72 min, respectively. The method was validated over a dynamic linear range of 50.0 to 150 μg/mL and 5.0 to 15.0 μg/mL for Vildagliptin and dapagliflozin, respectively, with a correlation coefficient of 0.999. The precision and Keywords: Vildagliptin, Dapagliflozin, Protein precipitation, Human plasma, RP-HPLC, Simultaneous determination. [Download Article] [Download Certifiate] |