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ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF MULTI-VITAMIN CAPSULES USING RP-HPLC METHOD
G. Deepa*, M. Karthik, R. Kanmani, P. D. Gokulan and K. L. Senthil Kumar
ABSTRACT
In the present study, we developed a reliable and robust chromatographic method for the quantification of multivitamins in tablet samples of Vit B1, B2, B3, B6 by Reverse Phase High- performance liquid chromatography (RP-HPLC) with photodiode array detection using C18 column (250 x 4.6 mm, 5μm)under the wave length 280nm. The vitamins nicotinamide, pyridoxine, riboflavin, and thiamin were analyzed and quantified in a total analysis time of 40 minutes, and individual retention time of Vit B1 is 36.29mins, Vit B2 18.32 mins, Vit B3 5.47 mins, Vit B6 is 10.28 mins using reversed-phase (RP)-HPLC. The method linearity exhibits a good correlation coefficient (R2 = 0.999). The method precision %RSD of Vit B1, B2, B3, B6 (0.6, 0.4, 0.3, 0.5), the accuracy %RSD of Vit B1, B2, B3, B6 Keywords: Reverse Phase High Performance Liquid Chromatography, Method development, validation, Vit B1, B2, B3, B6(nicotinamide, pyridoxine, riboflavin, and thiamine). [Download Article] [Download Certifiate] |