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A REVIEW ON GEL CHROMATOGRAPHY
Dr. M. Sri. Ramchandra* and Shaik Intikhaf Alam
ABSTRACT
Gel chromatography, also called gel filtration chromatography or size exclusion chromatography, is a widely used technique in biochemistry and molecular biology to separate and purify molecules based on their size and shape. It operates on the principle of differential partitioning between a stationary phase (composed of porous beads or gel particles) and a mobile phase (a buffer solution). The sample mixture is carried by the mobile phase through the gel bed, and different-sized molecules are separated by their ability to enter the pores of the gel beads. Smaller molecules spend less time in the stationary phase and elute faster, while larger molecules are excluded from entering the pores and elute slower. Gel chromatography is a gentle and non-denaturing method for biomolecule purification and analysis, as it allows for separation without compromising their structural and functional integrity. It is Keywords: Gel-filtration chromatography, Gel-permeation, Gel-exclusion, Size-exclusion, Molecular- sieve, Operating conditions, Separations, Molecular mass estimation, Size-exclusion reaction chromatography. [Download Article] [Download Certifiate] |