Abstract

RECENT TECHNOLOGICAL PROGRESS IN PROTEIN ANALYSIS THROUGH LC-MS METHODS

Vishakha Tyagi* and Yashvardhan Vats

ABSTRACT

The abstract highlights the significance of liquid chromatography-mass spectrometry (LC-MS) in protein analysis, emphasizing its sensitivity, accuracy, and versatility. The critical role of sample preparation in ensuring accurate mass spectrometry results, focusing on the removal of contaminants such as salts, detergents, and buffers. Various strategies for salt and detergent removal, including the utilization of reversed-phase HPLC columns and trap columns, are outlined to enhance sensitivity and sample purity. Additionally, the abstract discusses the bottom-up and top-down approaches for protein analysis via MS, highlighting their respective methodologies and applications. The importance of HPLC techniques in peptide separation and addresses ongoing challenges in proteomics research, such asimproving proteome coverage and managing method development costs. The abstract concludes by emphasizing the increasing relevance of HPLC/UHPLC-MS in omics sciences and clinical settings, despite associated challenges like high upfront costs and the absence of a universal chromatographic method in proteomics.

Keywords: LC-MS, Sample Preparation, Proteomics research, Techniques.