Abstract

PHYTOCHEMICAL INVESTIGATION AND SEPARATION OF BIOACTIVE COMPOUNDS PRESENT IN ETHANOLIC LEAF EXTRACT OF MURAYYA KOENIGII BY TLC AND COLUMN CHROMATOGRAPHY

T. Anju*, Aamina Farheen, D. Supraja, E. Vamsi Vinay, K. Jaya Deepika, M. Divya, SK. Saleema

ABSTRACT

Many medicinal plants were studied for their contents of phytochemicals. Murraya koenigii is one of these plants. This study, primarily aimed to carry out a preliminary phytochemical screening to detect the major classes of bioactive compounds presented in Murraya koenigii dry leaves. Ethanolic solvent was used to determine the best solvent that can be used for extraction and to perform thin layer chromatography profiling. TLC was carried out in Silica gel plates using 2 mobile phase protocols, Chloroform: Ethanol: Methanol (1:1:1) and n-butanol: Acetonitrile: Distilled water (12.5:3.7:2.5) using ethanol as a solvent. Phytochemical screening of Ethanolic extract showed the presence of Alkaloids, glycosides, flavonoids, tannins, phenols, saponins and steroids. As well as Thin Layer Chromatographic studies of the Murraya koenigii Ethanolic leaf extract constituted different coloured phytochemical compounds with
different Rf values. Ethanolic extract shows seven bands with Rf less than 1 for 24 hrs. Further Column chromatographic studies of Ethanolic extract using 2 solvent system protocols Chloroform: Ethanol: Methanol and n-butanol: Acetonitrile: Distilled water shows the better separation with ethanol and n-butanol solvents respectively. From these studies it was concluded that the Ethanolic extract for Murraya koenigii leaves contain a higher content of bioactive compounds and ethanol, n-butanol as a solvents shows effective separation of bioactive compounds, which can be used for further research on this plant.

Keywords: Murraya koenigii, thin layer chromatography, column chromatography, Ethanolic extract.