Abstract

DEVELOPMENT AND EVALUATION OF ENTERIC COATED GUAR GUM NANOPARTICLES FOR AMOEBIASIS

Singh Fulendra,* M. Senthil Kumar

ABSTRACT

The present study was aimed to know the eradication rate of antiprotozoal fixed drugs combination of Tinidazole (TDZ) and Norfloxacin (NOR). Guar gum nanoparticles (GG NPs) were prepared by cross linked with glutaraldehyde for the treatment of amoebiasis. A double step w/o/w cross linked emulsion method was used to prepare blank and drug loaded nanoparticles. Enteric coated was done by solvent evaporation method to protect the drugs in gastric fluids and achieve a targeted drug delivery. The characterization of coated and uncoated Guar gum nanoparticles was characterized by morphology by scanning electron microscopy (SEM), internal diameter in transmission electron microscope (TEM), average particle size in (Zeta seizer), Fourier Transform Infrared Spectrophotometry (FT-IR). The drug polymer interactions were checked by making physical mixture of polymer and drug in Differential scanning calorimetry (DSC). TDZ and NOR loading estimated by high performance liquid chromatography (HPLC) and in vitro drug release characterized. Regression coefficient (R2) analysis suggested the controlled released mechanism from the nanoparticles. In vivo eradication studies on female albino mice demonstrated comparative eradication of marketed formulation and nanoparticle formulation. These studies revealed that controlled release behavior of GG NPs could be a potential alternative pharmaceutical formulation in treatment of amoebiasis.

Keywords: scanning electron microscopy (SEM), Transmission electron microscope (TEM), average particle size in (Zeta seizer).