Abstract

FORMULATION AND EVALUATION OF POLYHERBAL GEL

Dr. Saurabh Dilip Bhandare, Pravin Arungiri Gosavi* and Dr. Vijay Dhondiram Wagh

ABSTRACT

In traditional Indian medicine, Ayurveda, remedies for various infectious diseases and ailments include azadirachta indica, adhatoda vasica, piper betle, ocimum tenuiflorum, and pongamia pinnata. Building on the traditional use of these herbs, this study was conducted to develop and assess a polyherbal gel containing extracts from azadirachta indica, adhatoda vasica, piper betle, ocimum tenuiflorum, and pongamia pinnata. Three gel formulations (A, B, and C) were prepared with ethanolic extracts of these herbs at concentrations of 0.1%, 0.3%, and 0.5%, respectively, in a base. The prepared formulations underwent evaluation for appearance, homogeneity, pH, viscosity, rheological properties, spreadability, skin irritation, patch test, and washability. Antimicrobial activity of the formulations wasdetermined using the disc plate method against S. aureus, B. subtilis, A. niger, and E. coli. The study results indicated that all formulations (A, B, and C) exhibited a superior zone of inhibition compared to the control. Notably, formulation C demonstrated the highest activity against the selected strains, likely due to its higher concentration of herbal extracts compared to formulations A and B. The polyherbal gel formulations demonstrated antimicrobial action, and this effectiveness is likely attributed to the synergistic interaction of the plant constituents present in the formulation.

Keywords: Antimicrobial activity, patch test, polyherbal gel, evalution test.