CHARACTERISATION AND FATTY ACID PROFILE OF CUCURBITA PEPO SEED OIL
1,2Omeh Yusuf S*., 1Ijioma V.U., 2Ugwu Okechukwu P.C. and 2Enechi O.C.
1Department of Biochemistry, Michael Okpara, University of Agriculture ,Umudike, Abia State, Nigeria.
2Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria.
ABSTRACT
Cucurbita pepo oil was extracted from samples of the Cucurbita pepo
seed using Soxhlet solvent extraction. The oil yield of sample was
38.47%. The chemical and physical properties of the oil were also
determined using standard methods. Fatty acid components were
determined using the Gas chromatographic technique. The
physicochemical analysis revealed brown coloured oil with relative
density of (0.88), cloud point of (100ºC), PH of (1.96) at 25ºC, Melting
point of (110ºC), unsaponifiable matters of (2.58mgKoH/g),
saponification value (159.89mgKOH-1), acid value (3.74mgKOH/g),
Iodine value (81.0) and Peroxide value (4.00mgEqKg-1). Gas-Liquid
Chromatography also revealed that the major Fatty acids were:
C14:O(0.16%), C16:O(20.33%), C10:O(10.70%), C18:1(11.40%) and
C18:2(57.40%). The saponification and Iodine values obtained in this
study shows the industrial potential of the oil, both in soap and coating industries. Also the
high concentration of unsaturated fatty-acids in this study is an indicative that Cucurbita pepo
oil could be heart friendly and therefore should be recommended for cardiovascular patients.
Further investigation on the nutritional, pharmacological and lipid characterisation of
Cucurbita pepo seed would form the basis of our future research.
Keywords: Extraction, Characterisation, Physiochemical analysis, Soxhlet Extraction and Cucurbita pepo seeds.
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